Composite

Part:BBa_K2638201:Design

Designed by: Jakob Zubek   Group: iGEM18_Bielefeld-CeBiTec   (2018-09-02)


OprC (TonB dependent copper transport porin, BBa_K2638200) with T7 promotor and RBS (BBa_K525998)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 290
    Illegal NgoMIV site found at 621
    Illegal NgoMIV site found at 825
    Illegal NgoMIV site found at 1869
    Illegal NgoMIV site found at 2070
    Illegal AgeI site found at 284
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The PstT restriction side had to be deleted from the original sequence.


Source

Pseudomonas brassicacearum 3Re2-7 13 BBa_K525998


References